Squalene in Pisum sativum

DNA methylase from Pisum sativum.

DNA methylase activity was detected in nuclei from pea shoots. The enzyme can only be extracted by low-salt treatment if the nuclei are pretreated with micrococcal nuclease. Only a single enzyme was detected, and it was purified to a specific activity of 1620 units/mg of protein. It has an Mr of 160,000 on gel filtration and SDS/PAGE. Pea DNA methylase methylates cytosine in all four dinucleoti...

The isoperoxidases of Pisum sativum.

The heterogeneity of the peroxidases in peas was examined by starch gel electrophoresis. Comparisons were made between tall and dwarf cultivars and among organ systems developed in light and darkness. Isoperoxidase bands could be grouped as cathodic, anodic and near-neutral (at pH 9.0) types. The cathodic set stained well with guaiacol oxidation products whereas some anodic bands reacted prefer...

Concerning artificial crossing in Pisum sativum.

Nucleic Acid Metabolism in Pisum sativum Alaska

Sunmmary. Phosfon-S, a substance which inhibits stem elongation, alters nucleic acid metaboli,sm in Pisutm sativum Alaska. Methylated albumin kieselguhr (MAK) columns were used to fractionate 32P-labeled nucleic acids. Phosfon-S treatment of the plants resulted in a decrease in soluble RNA and an increase in ribo,somal RNA. Specific activities of the various nucleic acid fractions were lower as...

Ribosomal DNA Amounts in PISUM SATIVUM.

Different varieties of peas have different proportions of rDNA in their genomes; there is no obvious correlation between the proportions and seed or leaf size. The rDNA proportions in root tips, seedlings, leaves and in the cotyledon cells of high DNA content, were compared in four varieties. In three, there was no difference between tissues; the fourth showed an amplification of rRNA genes in ...